skills

Omics
Proficient in sample preparation for whole exome seq, bulk RNA seq, single-cell RNA-seq, CITE-seq, and TCR/BCR-seq and its data analysis using R and Python.

Microscopy
Well seasoned in brightfield, phase-contrast, fluorescence, confocal, and multiplex tissue imaging. Experienced in Transmission Electron Microscopy (TEM) and super-resolution microscopy.

Tissue Processing
Expert in macro-dissection of tumour epithelium, preparation of FFPE blocks, tissue sectioning, haematoxylin and eosin staining (H&E), immunohistochemistry, micro-dissection of epithelial tissue for DNA isolation, and primary tumour-derived cell line generation.

3D Cell Culture
Experienced in generating spheroids and organoids, isolating and culturing immune cells, and establishing organoid–immune cell co-culture systems.

2D Cell Culture
Skilled in the maintenance and manipulation of human cell lines, including drug treatment, cell viability assays, transfection, viral transduction, stable clone generation, immunofluorescence assays, migration and wound healing assays.

Microarray
Hands-on experience with array-based Comparative Genomic Hybridization (aCGH) and gene expression microarrays. Competent in R programming for data preprocessing, normalization, clustering, CMS, SAM, and GSEA. Skilled in using online tools and databases such as cBioPortal, Enrichr, UCSC Genome Browser (ENCODE), Gene Ontology, and WebGestalt.

Molecular Biology
Proficient in DNA and RNA isolation from blood, frozen, and FFPE tissues; and in techniques like mutation screening, denaturing urea PAGE, formaldehyde agarose gel electrophoresis, reverse transcription, quantitative PCR (qPCR), molecular cloning, site-directed mutagenesis, chromatin immunoprecipitation (ChIP), and luciferase reporter assays.

Protein Biology
Experienced in protein extraction and purification, dialysis, SDS and native PAGE, and Western blotting for protein analysis.

Infection Biology
Hands-on experience in culturing clinical isolates of Leishmania donovani, infecting human cell lines, and performing related assays including DNA fragmentation, Giemsa staining, CFSE and PI staining. Trained in Biosafety Level 2 (BSL-2) facility for SARS-CoV-2 diagnostics.

Model Organisms
Experienced in dissection and handling of BALB/cJ and C57BL/6J mice. Familiar with Drosophila melanogaster techniques through advanced training. Certified in “Basics of Mouse Genetics” and “Introduction to CRISPR/Cas9 and Cre-lox Technologies” from The Jackson Laboratory, USA.

Biostatistics and Epidemiology
Trained in clinical study design, collection and analysis of clinicopathological and survival data, hypothesis testing, and data visualization.